Recombinant Cytokeratin 18 anticorps

N° du produit ABIN6937676

Cet anticorps Souris Monoclonal détecte spécifiquement Cytokeratin 18 dans WB, IHC, IF et FACS. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Chien, Porc, Cat, Boeuf (Vache), Hamster, Mouton, Cheval, Chévre, Furet et Marmoset.

Aperçu rapide pour Recombinant Cytokeratin 18 anticorps (ABIN6937676)

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat, Chien, Porc, Cat, Boeuf (Vache), Hamster, Mouton, Cheval, Chévre, Furet, Marmoset

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 18 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: RKRT18-1190

Détail du produit anti-Cytokeratin 18 anticorps

Fonction: Recombinant Cytokeratin 18 (KRT18) Antibody

Specificité: This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Aucune reactivité croisée: Poulet, Xenopus laevis

Réactivité croisée (Details): Human. Mouse. Rat. Hamster. Ferret. Cow. Pig. Cat. Dog. Sheep. Goat. Horse. Marmoset monkey. Does not react with Chicken or Xenopus.

Purification: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogène: Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 or HeLa cells (FACS/IF). Human breast carcinoma tissue (IHC).

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Cytokeratin 18

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Autre désignation: KRT18

Sujet:

Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18

MCF-7 or HeLa cells (FACS/IF). Human breast carcinoma tissue (IHC). Human Intestine, human Lung, Human Skin, A431, HCT116, HeLa.

Poids moléculaire: 45kDa

ID gène: 3875, 406013

UniProt: P05783

Pathways: Apoptose, Caspase Cascade in Apoptosis