Cytokeratin 18 anticorps

N° du produit ABIN6937677

L’anticorps Souris Monoclonal anti-Cytokeratin 18 a été validé pour WB, IF, FACS et IHC (f). Il convient pour détecter Cytokeratin 18 dans des échantillons de Humain.

Aperçu rapide pour Cytokeratin 18 anticorps (ABIN6937677)

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 18 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: DC10

Détail du produit anti-Cytokeratin 18 anticorps

Specificité: This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Aucune reactivité croisée: Boeuf (Vache), Chien, Furet, Hamster, Souris, Porc, Rat (Rattus), Mouton

Réactivité croisée (Details): Human. Does not react with Mouse, Rat, Ferret, Hamster, Sheep, Cow, Dog or Pig.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: PMC-42 human breast carcinoma cells

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, HeLa, A431 cells. Breast Cancer.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur Cytokeratin 18

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Autre désignation: KRT18

Sujet: Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18,Cytokeratin 18 (KRT18)
Cellular localisation: Cytoplasmic

Poids moléculaire: 45kDa

ID gène: 3875, 406013

UniProt: P05783

Pathways: Apoptose, Caspase Cascade in Apoptosis