KRT8, KRT18 anticorps

N° du produit ABIN6937758

Détail du produit

Antigène: KRT8, KRT18

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificité: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.

Aucune reactivité croisée: Poulet, Âne, Furet, Souris, Lapin, Rat (Rattus), Xenopus laevis

Réactivité croisée (Details): Human. Monkey. Cow. Sheep. Pig. Does not react with Mouse, Rat, Ferret, Rabbit, donkey, Chicken or xenopus laevis.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Cytoskeleton preparation from HeLa cells

Clone: C-51

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détail du antigène

Antigène: KRT8, KRT18

Autre désignation: KRT8 & KRT18

Sujet: CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18,Cytokeratin 8/18
Cellular localisation: Cytoplasmic

Poids moléculaire: 52.5kDa (CK8), 45kDa (CK18)

ID gène: 3856, 3875, 533782

UniProt: P05787, P05783