KRT8, KRT18 anticorps

N° du produit ABIN6941351

Détail du produit

Antigène: KRT8, KRT18

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Specificité: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immuno-histochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.

Purification: Purified by Protein A/G

Immunogène: Recombinant full-length human KRT8 protein (KRT8/803), Recombinant full-length human KRT18 protein (KRT18/835)

Clone: KRT8-803-KRT18-835

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détail du antigène

Antigène: KRT8, KRT18

Autre désignation: KRT8 & KRT18

Poids moléculaire: 52.5kDa (CK8), 45kDa (CK18)

ID gène: 3856, 3875

UniProt: P05787, P05783