LIG1 anticorps

N° du produit ABIN6937786

Cet anticorps anti-LIG1 est un anticorps Souris Monoclonal détectant LIG1 dans WB, ELISA et IP. Adapté pour Boeuf (Vache).

Aperçu rapide pour LIG1 anticorps (ABIN6937786)

Antigène: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivité: Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LIG1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: 1A9

Détail du produit anti-LIG1 anticorps

Specificité: DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. Furthermore, DNA Ligase I antisense ODNs syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme.

Réactivité croisée (Details): Human. Mouse. Rat. Bovine.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Full-length native cow LIG1 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Western Blot (1-2 μg/mL), ELISA, Immunoprecipitation,Optimal dilution for a specific application should be determined.

Positive_Control: MOLT-4, PC-3 or Jurkat cell lysates.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur LIG1

Antigène: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Autre désignation: LIG1

Sujet: DNA ligase 1, DNA ligase I, DNLI1_HUMAN, LIG 1, lig1, Ligase I DNA ATP dependent, MGC117397, MGC130025, Polydeoxyribonucleotide synthase [ATP] 1,DNA Ligase 1
Cellular localisation: Nucleus.

Poids moléculaire: 133kDa

ID gène: 3978, 1770

UniProt: P18858

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA