MSH6 anticorps (AA 374-540)

N° du produit ABIN6937906

Cet anticorps anti-MSH6 Monoclonal Souris (Clone MSH6-3086) (ABIN6937906) détecte spécifiquement MSH6 dans WB, IHC, IF, ELISA et FACS. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour MSH6 anticorps (AA 374-540) (ABIN6937906)

Antigène: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MSH6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)

Clone: MSH6-3086

Détail du produit anti-MSH6 anticorps

Épitope: AA 374-540

Fonction: MSH6 (DNA Mismatch Repair Protein) Antibody

Specificité: The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.

Réactivité croisée (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment of human MSH6 protein (around aa 374-540) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Information d'application

Indications d'application:

Known_Application: ELISA (For coating, order Ab without BSA), Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HCT116 or HeLa cell lysate (WB). Human colon carcinoma (IHC).

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur MSH6

Antigène: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Autre désignation: MSH6

Sujet:

DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, mutS (E. coli) homolog 6, MutS alpha 160 kDa subunit, MutS-alpha 160 kDa subunit, p160, Sperm associated protein

HCT116 or HeLa cell lysate (WB). Human colon carcinoma (IHC). HEK293

Poids moléculaire: 163kDa

ID gène: 2956, 445052

UniProt: P52701

Pathways: Réparation de l'ADN, Chromatin Binding, Production of Molecular Mediator of Immune Response