NPM1 anticorps (AA 185-287)
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- Antigène Voir toutes NPM1 Anticorps
- NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))
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Épitope
- AA 185-287
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp NPM1 est non-conjugé
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Application
- ELISA, Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificité
- Recognizes a 33 kDa glycoprotein, identified as Nucleophosmin (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.
- Réactivité croisée (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogène
- Recombinant human NPM1 protein fragment (aa185-287) (exact sequence is proprietary)
- Clone
- NPM1-3285
- Isotype
- IgG2c kappa
- Top Product
- Discover our top product NPM1 Anticorps primaire
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- Indications d'application
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Known_Application: Immunofluorescence (1-2 μg/mL), ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: K562, MCF-7, HeLa or A431 cells. Skin, Colon.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Agent conservateur
- Azide free
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Date de péremption
- 24 months
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- Antigène
- NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))
- Autre désignation
- NPM1 (NPM1 Produits)
- Synonymes
- anticorps B23, anticorps NPM, anticorps NO38, anticorps Npm, anticorps B23NP, anticorps npm4, anticorps npm, anticorps CG7917, anticorps CRP1, anticorps Crp1, anticorps Dmel\\CG7917, anticorps dNLP, anticorps dNLP-S, anticorps p22, anticorps npm1, anticorps sb:cb487, anticorps nucleophosmin 1, anticorps nucleophosmin (nucleolar phosphoprotein B23, numatrin) L homeolog, anticorps nucleophosmin 1b, anticorps Nucleophosmin, anticorps nucleophosmin, anticorps nucleophosmin (nucleolar phosphoprotein B23, numatrin), anticorps Nucleoplasmin, anticorps nucleophosmin 1a, anticorps NPM1, anticorps Npm1, anticorps npm1.L, anticorps npm1b, anticorps npm, anticorps Nlp, anticorps npm1a
- Sujet
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NO38, NPM, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin (nucleolar phosphoprotein B23 numatrin), Nucleophosmin, Nucleophosmin/nucleoplasmin family member 1, Numatrin,Nucleophosmin (Acute Myeloid Leukemia Marker)
Cellular localisation: Nuclear and Cytoplasmic - Poids moléculaire
- 33kDa
- ID gène
- 4869, 557550
- UniProt
- P06748
- Pathways
- Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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