CD31 anticorps

N° du produit ABIN6938149

Cet anticorps anti-CD31 Monoclonal Souris (Clone SPM122) (ABIN6938149) détecte spécifiquement CD31 dans FACS, WB, IHC et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CD31 anticorps (ABIN6938149)

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD31 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: SPM122

Détail du produit anti-CD31 anticorps

Fonction: CD31 / PECAM-1 (Endothelial Cell Marker) Antibody

Specificité: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Aucune reactivité croisée: Porc, Rat (Rattus)

Réactivité croisée (Details): Human. Cynomolgus Monkey. Rabbit. Does not react with Rat or Pig.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Membrane preparation of a spleen from a patient with hairy cell leukemia

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Tonsil, Angiosarcoma.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD31

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Autre désignation: PECAM1

Sujet:

EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA'

Jurkat cells. Human tonsil.

Poids moléculaire: ~100kDa (endothelium) and ~130kDa (platelets)'

ID gène: 5175, 376675, 514412

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization