Recombinant TNC anticorps

N° du produit ABIN6938600

Cet anticorps Souris Monoclonal détecte spécifiquement TNC dans IHC et IHC (f). Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant TNC anticorps (ABIN6938600)

Antigène: TNC (Tenascin C (TNC))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TNC est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: TNC-3635

Détail du produit anti-TNC anticorps

Specificité: In Western blotting, it reacts with two bands of ~MW of 210 kDa and 300 kDa, identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

Aucune reactivité croisée: Rat (Rattus)

Réactivité croisée (Details): Human. Does not react with rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogène: Recombinant human full-length TNC protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Known_Application: Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) ,Optimal dilution for a specific application should be determined.

Positive_Control: Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast, squamous cell, and lung carcinomas. Staining of normal fibroblasts serves as internal positive control (IHC).

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Agent conservateur: Azide free

Stock: -20 °C,-80 °C

Stockage commentaire: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Date de péremption: 24 months

Détails sur TNC

Antigène: TNC (Tenascin C (TNC))

Autre désignation: TNC

Sujet: Cytotactin, Glioma-associated-extracellular matrix antigen, GMEM, GP 150-225, Hexabrachion (HXB), JI, Myotendinous antigen, Neuronectin, TNC,Tenascin C (Stromal Marker for Epithelial Malignancy)
Cellular localisation: Connective tissue matrix

Poids moléculaire: 210kDa and 300kDa

ID gène: 3371, 143250

UniProt: P24821

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development