CDK2 anticorps

N° du produit ABIN6939030

Cet anticorps Souris Monoclonal détecte spécifiquement CDK2 dans WB, IP, FACS, Act et KAA. Il présente une réactivité envers Humain.

Aperçu rapide pour CDK2 anticorps (ABIN6939030)

Antigène: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDK2 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Activation (Act), Kinase Activity Assay (KAA)

Clone: AN4-3

Détail du produit anti-CDK2 anticorps

Specificité: In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclindependent kinase 2 (Cdk2), also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2-related cyclin dependent kinases have been identified. These include Cdk3, Cdk4, Cdk5, PCTAIRE-1, PCTAIRE-2, PCTAIRE-3, Cdk6 Cdk7, Cdk8 and KKIALRE.

Réactivité croisée (Details): Human, Mouse, Xenopus,

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant full-length human Cdk2 protein

Isotype: IgG2a

Information d'application

Indications d'application:

Positive Control: HeLa cells. HeLa whole cell lysate.

Known Application: Inhibits activation of p34cdc2 kinase by cyclins, Immunoprecipitation, Kinase Assay, ,Western Blot, Flow cytometry (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Date de péremption: 24 months

Détails sur CDK2

Antigène: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Autre désignation: CDK2

Sujet: Cdc2 related protein kinase, CDC28, CDC2A, CDK1, CDK2, CDKN2, Cell devision kinase 2, Cell division protein kinase 2, cyclin dependent kinase 2 alpha, kinase Cdc2, MPF, p33 protein kinase,Cdk2 / p34cdc2 Serine-Threonine Kinase
Cellular localisation: Nuclear

Poids moléculaire: 34kDa

ID gène: 1017, 19192

UniProt: P24941

Pathways: Signalisation PI3K-Akt, Cycle Cellulaire, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA