CHD4 anticorps (C-Term)

N° du produit ABIN6939119

Cet anticorps Souris Monoclonal détecte spécifiquement CHD4 dans WB, ELISA, IF, FACS, Coat et IHC (f). Il présente une réactivité envers Synthetic.

Aperçu rapide pour CHD4 anticorps (C-Term) (ABIN6939119)

Antigène: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Reactivité: Synthetic

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CHD4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 3F2-4

Détail du produit anti-CHD4 anticorps

Épitope: C-Term

Specificité: CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function primarily in transcriptional repression. Accumulating evidence indicates that NuRD may regulate the transcription of specific genes by interacting with specific transcriptional factors. In addition, the NuRD complex may also participate in genome-wide transcriptional regulation through an association with histone tails.

Réactivité croisée (Details): Human, Mouse,

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Synthetic peptide (N- and C- terminal peptides were used for immunisation: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ) - which peptide the antibody recognises has not been tested.

Isotype: IgG2a

Information d'application

Indications d'application:

Positive Control: HeLa whole cell lysate or human placenta tissue lysate. Human breast carcinoma.

Known Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL)Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Date de péremption: 24 months

Détails sur CHD4

Antigène: CHD4 (Chromodomain Helicase DNA Binding Protein 4 (CHD4))

Autre désignation: CHD4

Sujet: ATP dependent helicase CHD4, Chromodomain helicase DNA binding protein 4, Mi 2 autoantigen 218 kDa protein, Mi 2b, Mi2 beta,CHD4
Cellular localisation: Nucleus and cytoplasm.

Poids moléculaire: 238kDa

ID gène: 1108, 162233

UniProt: Q14839