CLTA/CLTB anticorps (N-Term)

N° du produit ABIN6939151

L’anticorps Souris Monoclonal anti-CLTA/CLTB a été validé pour WB, FACS, IF, IHC et StM. Il convient pour détecter CLTA/CLTB dans des échantillons de Humain.

Aperçu rapide pour CLTA/CLTB anticorps (N-Term) (ABIN6939151)

Antigène: CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CLTA/CLTB est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: SPM174

Détail du produit anti-CLTA/CLTB anticorps

Épitope: N-Term

Specificité: Recognizes proteins of 31-44 kDa, which are identified as Clathrin Light Chains (both A B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60 % homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.

Purification: Purified by Protein A/G

Immunogène: Purified recombinant N-terminal fragment of human Clathrin Light Chain

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Positive Control: HeLa cells. Placenta or Prostate Carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CLTA/CLTB

Antigène: CLTA/CLTB (Clathrin, Light Chain A/B (CLTA/CLTB))

Autre désignation: CLTA and CLTB

Poids moléculaire: 31-44kDa

ID gène: 1211, 1212

UniProt: P09496, P09497