CD21 anticorps (AA 142-240)

N° du produit ABIN6939184

Cet anticorps Souris Monoclonal détecte spécifiquement CD21 dans FACS, IHC, WB, IF, ELISA, StM et Coat. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour CD21 anticorps (AA 142-240) (ABIN6939184)

Antigène: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD21 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), ELISA, Staining Methods (StM), Coating (Coat)

Clone: CR2-2754

Détail du produit anti-CD21 anticorps

Épitope: AA 142-240

Specificité: Recognizes a protein of 140 kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment (around aa 142-240) of human CR2 (CD21) protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: MOLT4, Raji or WEHI-231 cells. Tonsil.

Known Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells),Western Blot (1-2 μg/mL),Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD21

Antigène: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Autre désignation: CR2

Poids moléculaire: 140kDa

ID gène: 1380

UniProt: P20023

Pathways: Système du Complément