ERCC1 anticorps (AA 191-281)

N° du produit ABIN6939333

Cet anticorps Souris Monoclonal détecte spécifiquement ERCC1 dans IHC et StM. Il présente une réactivité envers Humain.

Aperçu rapide pour ERCC1 anticorps (AA 191-281) (ABIN6939333)

Antigène: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ERCC1 est non-conjugé

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: ERCC1-2318

Détail du produit anti-ERCC1 anticorps

Épitope: AA 191-281

Specificité: Recognizes a protein of 110 kDa, identified as Excision Repair Cross Complementing 1 (ERCC1). It is a mammalian nucleotide excision repair (NER) enzyme involved in repair of damaged DNA. ERCC1 is a homologous to RAD10 in Saccharomyces cerevisiae, which is required in mitotic intrachromosomal recombination and repair. ERCC1 is required in repair of cisplatin-induced DNA adducts and ultraviolet (UV)-induced DNA damage. High expression of ERCC1 has been linked to tumor progression in a variety of cancers including non-small cell lung cancer (NSCLC), squamous cell carcinoma of the head, ovarian cancer and esophageal cancer.

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment (around aa 191-281) of human ERCC1 protein (exact sequence is proprietary)

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Positive Control: HeLa, HepG2 cells. Tonsil or Prostate.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur ERCC1

Antigène: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Autre désignation: ERCC1

Poids moléculaire: 110kDa

ID gène: 2067

UniProt: P07992

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response