FAT1 anticorps (Cytoplasmic Domain)

N° du produit ABIN6939370

Cet anticorps anti-FAT1 est un anticorps Souris Monoclonal détectant FAT1 dans ELISA, WB, Coat et IF. Adapté pour Drosophila melanogaster.

Aperçu rapide pour FAT1 anticorps (Cytoplasmic Domain) (ABIN6939370)

Antigène: FAT1 (FAT Tumor Suppressor Homolog 1 (FAT1))

Reactivité: Drosophila melanogaster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FAT1 est non-conjugé

Application: ELISA, Western Blotting (WB), Coating (Coat), Immunofluorescence (IF)

Clone: FAT1-3D7-1

Détail du produit anti-FAT1 anticorps

Épitope: Cytoplasmic Domain

Réactivité croisée (Details): Expected to cross-react with Chimpanzee. Rat (92 % . 85 % identity with immunogen. respectively) due to sequence homology.

Purification: Purified by Protein A/G

Immunogène: Cytoplasmic domain of Drosophila Fat protein.

Isotype: IgM kappa

Information d'application

Indications d'application:

Positive Control: Wild type imaginal discs from third instar Drosophila larvae.

Known Application: ELISA (For coating, order antibody without BSA), Western Blot (0.5-1.0 μg/mL), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur FAT1

Antigène: FAT1 (FAT Tumor Suppressor Homolog 1 (FAT1))

Autre désignation: FAT1

Sujet: The FAT proteins are members of the Cadherin superfamily homologous to the Drosophila Fat protein that functions as a positive regulator of planar cell polarity in the Drosophila wing. FAT1 is an unusual cadherin that controls cell growth and planar polarity while acting as a tumor suppressor. FAT1 is a proximal element of a signaling pathway that determines both cellular polarity in the plane of the monolayer and directed actin-dependent cell motility. FAT1 is localized at the leading edge of lamellipodia, filopodia and microspike tips where it directly interacts with Ena/VASP proteins to regulate the actin polymerization complex. When targeted to mitochondrial outer leaflets, the cytoplasmic domain of FAT1 recruits components of the actin polymerization machinery sufficient to induce ectopic actin polymerization. FAT1 expression in vascular smooth muscle cells (VSMCs) increases significantly after arterial injury or growth factor stimulation, implicating FAT1 in the control of VSMC functions central to vascular remodeling by facilitating migration and limiting proliferation. FAT1 is also involved in psychic disorders, and its action may be of patho-physiological importance.

Poids moléculaire: 500kDa

ID gène: 2195

UniProt: Q14517