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Fibronectin 1 anticorps

L’anticorps Souris Monoclonal anti-Fibronectin 1 a été validé pour IHC, IF, FACS et StM. Il convient pour détecter Fibronectin 1 dans des échantillons de Humain.
N° du produit ABIN6939413

Aperçu rapide pour Fibronectin 1 anticorps (ABIN6939413)

Antigène

Voir toutes Fibronectin 1 (FN1) Anticorps
Fibronectin 1 (FN1)

Reactivité

  • 94
  • 35
  • 31
  • 7
  • 6
  • 6
  • 5
  • 5
  • 4
  • 3
  • 3
  • 1
  • 1
  • 1
Humain

Hôte

  • 77
  • 30
  • 1
  • 1
Souris

Clonalité

  • 74
  • 34
Monoclonal

Conjugué

  • 63
  • 12
  • 10
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Fibronectin 1 est non-conjugé

Application

  • 70
  • 50
  • 25
  • 15
  • 13
  • 13
  • 12
  • 9
  • 8
  • 7
  • 7
  • 6
  • 6
  • 1
  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone

568
  • Specificité

    Epitope of this MAb is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.

    Purification

    Purified by Protein A/G

    Immunogène

    High molecular weight proteins secreted by cultivated human fibroblasts

    Isotype

    IgG1 kappa
  • Indications d'application

    Positive Control: SW156 cells. Kidney.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions

    For Research Use only
  • Concentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-80 °C

    Stockage commentaire

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Date de péremption

    24 months
  • Antigène

    Fibronectin 1 (FN1)

    Autre désignation

    FN1

    Poids moléculaire

    220kDa (monomer), 440kDa (dimer)

    ID gène

    2335

    UniProt

    P02751

    Pathways

    Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy
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