Recombinant Elastase 3B anticorps (AA 82-238)

N° du produit ABIN6939416

Cet anticorps anti-Elastase 3B est un anticorps Souris Monoclonal détectant Elastase 3B dans WB, IHC et StM. Adapté pour Humain.

Aperçu rapide pour Recombinant Elastase 3B anticorps (AA 82-238) (ABIN6939416)

Antigène: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Elastase 3B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: RCELA3B-1811

Détail du produit anti-Elastase 3B anticorps

Épitope: AA 82-238

Specificité: This MAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B). Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function.

Purification: Purified by Protein A/G

Immunogène: Recombinant human CELA3B protein fragment (around aa 82-238) (exact sequence is proprietary)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Panc-28 or PANC1 cells. Human Pancreas.

Known Application: Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Elastase 3B

Antigène: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Autre désignation: CELA3B

Poids moléculaire: ~17kDa

ID gène: 23436

UniProt: P08861