Recombinant AMACR anticorps (AA 297-394)

N° du produit ABIN6939432

L’anticorps Lapin Monoclonal anti-AMACR a été validé pour IHC et StM. Il convient pour détecter AMACR dans des échantillons de Humain.

Aperçu rapide pour Recombinant AMACR anticorps (AA 297-394) (ABIN6939432)

Antigène: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp AMACR est non-conjugé

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: AMACR-2748R

Détail du produit anti-AMACR anticorps

Épitope: AA 297-394

Specificité: This antibody recognizes a protein of 42 kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and -oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.

Purification: Purified by Protein A/G

Immunogène: Recombinant human AMACR protein fragment (around aa 297-394) (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application:

Positive Control: HEK cells. Prostate Adenocarcinoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur AMACR

Antigène: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Autre désignation: AMACR

Poids moléculaire: 42kDa

ID gène: 23600

UniProt: Q9UHK6

Pathways: Monocarboxylic Acid Catabolic Process