14-3-3 sigma/SFN anticorps

N° du produit ABIN6939540

Détail du produit anti-14-3-3 sigma/SFN anticorps

Antigène: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp 14-3-3 sigma/SFN est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogène: Recombinant human full-length SFN protein

Clone: CPTC-SFN-2

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: PC-3 cells, Skin and tissues enriched in stratified squamous keratinizing epithelium. HaCaT, hTCEpi, A-431 cell lines.

Known Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur 14-3-3 sigma/SFN

Antigène: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Autre désignation: SFN (SFN Produits)

Synonymes: anticorps YWHAS, anticorps Er, anticorps Mme1, anticorps Ywhas, anticorps stratifin, anticorps SFN, anticorps Sfn

Sujet: SFN (stratifin) is a p53-induced tumor suppressor gene which is activated in response to DNA damage, causing cell cycle arrest at G2 phase by blocking cdc2-cyclin B1 complex from entering the nucleus. It is inactivated in breast, lung, prostate, liver and gastric cancer. It is associated with poor prognosis when its down-regulation is observed in epithelial ovarian cancer. SFN expression could contribute to cancer cell proliferation and the development and/or progression of human gastrointestinal cancer.

Poids moléculaire: 28kDa

ID gène: 2810

UniProt: P31947

Pathways: Signalisation p53, Myometrial Relaxation and Contraction