Recombinant HSPD1 anticorps

N° du produit ABIN6939669

Cet anticorps anti-HSPD1 est un anticorps Souris Monoclonal détectant HSPD1 dans WB, IHC, ISt et StM. Adapté pour Humain, Souris, Rat, Poulet, Singe, Hamster, Chien, Boeuf (Vache), Lapin, Porc, Mouton, Drosophila melanogaster et Xenopus laevis.

Aperçu rapide pour Recombinant HSPD1 anticorps (ABIN6939669)

Antigène: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat, Poulet, Singe, Hamster, Chien, Boeuf (Vache), Lapin, Porc, Mouton, Drosophila melanogaster, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HSPD1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Clone: RGROEL-780

Détail du produit anti-HSPD1 anticorps

Specificité: Recognizes a 60 kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp's), more appropriately called as stress response proteins (srp's). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone rGROEL/780, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.

Aucune reactivité croisée: Bactéries, Nematode, Épinards (Spinacia oleracea)

Purification: Purified by Protein A/G

Immunogène: Recombinant full-length human HSP60 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.

Known Application: Western Blot (0.25-0.5 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur HSPD1

Antigène: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Autre désignation: HSPD1

Poids moléculaire: 60kDa

ID gène: 3329

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity