Recombinant Cytokeratin 18 anticorps

N° du produit ABIN6939938

Cet anticorps Lapin Monoclonal détecte spécifiquement Cytokeratin 18 dans WB, IHC, IF, FACS, StM et ISt. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant Cytokeratin 18 anticorps (ABIN6939938)

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Cytokeratin 18 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM), Immunostaining (ISt)

Clone: KRT18-2808R

Détail du produit anti-Cytokeratin 18 anticorps

Specificité: This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Purification: Purified by Protein A/G

Immunogène: Recombinant human full-length KRT18 protein

Isotype: IgG

Information d'application

Indications d'application:

Positive Control: HCT116, MCF-7, HeLa, A431 cells. Breast Cancer.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Cytokeratin 18

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Autre désignation: KRT18

Poids moléculaire: 45kDa

ID gène: 3875

UniProt: P05783

Pathways: Apoptose, Caspase Cascade in Apoptosis