LIG1 anticorps

N° du produit ABIN6939965

Cet anticorps anti-LIG1 est un anticorps Souris Monoclonal détectant LIG1 dans WB, ELISA et IP. Adapté pour Humain, Rat, Souris et Boeuf (Vache).

Aperçu rapide pour LIG1 anticorps (ABIN6939965)

Antigène: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivité: Humain, Rat, Souris, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LIG1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: 1A9

Détail du produit anti-LIG1 anticorps

Purification: Purified by Protein A/G

Immunogène: Full-length native cow LIG1 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: MOLT-4, PC-3 or Jurkat cell lysates.

Known Application: Western Blot (0.5-1 μg/mL), ELISA, Immunoprecipitation,Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur LIG1

Antigène: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Autre désignation: LIG1

Sujet: DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. Furthermore, DNA Ligase I antisense ODNs syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme.

Poids moléculaire: 133kDa

ID gène: 3978

UniProt: P18858

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA