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CD73 anticorps

NT5E Reactivité: Humain WB, FACS, IHC, IF, StM Hôte: Souris Monoclonal NT5E-2503 unconjugated
N° du produit ABIN6940227
  • Antigène Voir toutes CD73 (NT5E) Anticorps
    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
    Reactivité
    • 119
    • 59
    • 27
    • 18
    • 18
    • 16
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 92
    • 39
    • 17
    • 2
    Souris
    Clonalité
    • 83
    • 67
    Monoclonal
    Conjugué
    • 62
    • 18
    • 14
    • 11
    • 10
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CD73 est non-conjugé
    Application
    • 95
    • 64
    • 59
    • 51
    • 19
    • 19
    • 14
    • 14
    • 14
    • 8
    • 7
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)
    Purification
    Purified by Protein A/G
    Immunogène
    Recombinant full length human NT5E protein
    Clone
    NT5E-2503
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product NT5E Anticorps primaire
  • Indications d'application

    Positive Control: Tonsil, Prostate or Lung Carcinoma.

    Known Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Date de péremption
    24 months
  • Antigène
    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
    Autre désignation
    NT5E (NT5E Produits)
    Synonymes
    anticorps CALJA, anticorps CD73, anticorps E5NT, anticorps NT, anticorps NT5, anticorps NTE, anticorps eN, anticorps eNT, anticorps zgc:63784, anticorps NT5E, anticorps MGC114869, anticorps PSPTO2277, anticorps 2210401F01Rik, anticorps AI447961, anticorps Nt5, anticorps 5'-nucleotidase ecto, anticorps 5'-nucleotidase, ecto (CD73), anticorps 5'-nucleotidase, anticorps 5' nucleotidase, ecto, anticorps NT5E, anticorps nt5e, anticorps nt5e.S, anticorps PSPTO_2277, anticorps CBU_1233, anticorps MCA1322, anticorps Nt5e
    Sujet
    CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.
    Poids moléculaire
    71kDa
    ID gène
    4907
    UniProt
    P21589
    Pathways
    Synaptic Membrane, Ribonucleoside Biosynthetic Process
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