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Recombinant Cytochrome C anticorps (AA 1-80)

CYCS Reactivité: Humain, Rat, Souris, Cheval, Chien, Pigeon, Drosophila melanogaster, Grenouille IHC, StM Hôte: Souris Monoclonal rCYCS-1010 unconjugated Recombinant Antibody
N° du produit ABIN6940324
  • Antigène Voir toutes Cytochrome C (CYCS) Anticorps
    Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
    Type d'anticorp
    Recombinant Antibody
    Épitope
    • 16
    • 12
    • 10
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-80
    Reactivité
    • 146
    • 116
    • 96
    • 45
    • 31
    • 23
    • 21
    • 19
    • 17
    • 15
    • 7
    • 7
    • 6
    • 6
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Rat, Souris, Cheval, Chien, Pigeon, Drosophila melanogaster, Grenouille
    Hôte
    • 86
    • 75
    • 7
    • 1
    Souris
    Clonalité
    • 88
    • 80
    • 1
    Monoclonal
    Conjugué
    • 105
    • 13
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Cytochrome C est non-conjugé
    Application
    • 137
    • 51
    • 43
    • 39
    • 35
    • 33
    • 27
    • 25
    • 14
    • 14
    • 13
    • 13
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Staining Methods (StM)
    Specificité
    It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
    Purification
    Purified by Protein A/G
    Immunogène
    Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c
    Clone
    RCYCS-1010
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product CYCS Anticorps primaire
  • Indications d'application

    Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

    Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-80 °C
    Stockage commentaire
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Date de péremption
    24 months
  • Antigène
    Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
    Autre désignation
    CYCS (CYCS Produits)
    Synonymes
    anticorps CYC, anticorps HCS, anticorps THC4, anticorps cyct, anticorps cyc, anticorps hcs, anticorps CYCS, anticorps cytc, anticorps CYCSA, anticorps CG17903, anticorps Cyt C, anticorps Cyt c, anticorps Cyt-c2, anticorps CytC-2, anticorps Cytc, anticorps Cytc-p, anticorps DC4, anticorps DMc01, anticorps Dmel\\CG17903, anticorps cyt c, anticorps cyt c-p, anticorps cyt-c-p, anticorps cyt-c2, anticorps cyt.c, anticorps cytochrome c, anticorps dc4, anticorps pDMc01, anticorps wu:fk52b01, anticorps zgc:86706, anticorps ENSMUSG00000058927, anticorps cytochrome c, somatic, anticorps cytochrome c, somatic S homeolog, anticorps Cytochrome c proximal, anticorps cytochrome c, somatic b, anticorps cytochrome c, somatic pseudogene, anticorps cytochrome c-like, anticorps cytochrome c, anticorps CYCS, anticorps cycs.S, anticorps cycs, anticorps Cycs, anticorps Cyt-c-p, anticorps cycsb, anticorps Gm10053, anticorps LOC100724240, anticorps CYC, anticorps LOC100847700, anticorps LOC101107954, anticorps LOC106829744, anticorps LOC106992390, anticorps LOC102085712
    Poids moléculaire
    15kDa
    ID gène
    54205
    UniProt
    P99999
    Pathways
    Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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