S100A8/A9 Complex (Calprotectin) anticorps

N° du produit ABIN6940547

Cet anticorps anti-S100A8/A9 Complex (Calprotectin) est un anticorps Souris Monoclonal détectant S100A8/A9 Complex (Calprotectin) dans IHC, FACS, IF et StM. Adapté pour Humain, Porc, Boeuf (Vache), Cat, Chien, Cobaye, Singe, Lapin, Rat, Souris, Cheval, Babouin et Chévre.

Aperçu rapide pour S100A8/A9 Complex (Calprotectin) anticorps (ABIN6940547)

Antigène: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Reactivité: Humain, Porc, Boeuf (Vache), Cat, Chien, Cobaye, Singe, Lapin, Rat, Souris, Cheval, Babouin, Chévre

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp S100A8/A9 Complex (Calprotectin) est non-conjugé

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Clone: SPM281

Détail du produit anti-S100A8/A9 Complex (Calprotectin) anticorps

Specificité: Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12 kDa alpha chain and a 14 kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.

Purification: Purified by Protein A/G

Immunogène: Affinity Purified monocyte membrane preparation

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Tonsil, lymph node or spleen.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur S100A8/A9 Complex (Calprotectin)

Antigène: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Autre désignation: S100A8 & S100A9

Poids moléculaire: 12-14kDa (doublet)

ID gène: 6279, 6280

UniProt: P05109, P06702

Pathways: S100 Proteins