Melanoma gp100 anticorps (AA 376-502)

N° du produit ABIN6940589

Cet anticorps Souris Monoclonal détecte spécifiquement Melanoma gp100 dans IHC, WB et ELISA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Melanoma gp100 anticorps (AA 376-502) (ABIN6940589)

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Melanoma gp100 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Clone: PMEL-2037

Détail du produit anti-Melanoma gp100 anticorps

Épitope: AA 376-502

Fonction: gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Antibody

Specificité: The gp100 Molecule is a 100 kDa glycosylated protein that is cleaved into a small (26 kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26 kDa and 34-38 kDa fragments. Cytotoxic T lymphocytes (CTL s) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein.

Purification: 200ug/ml of Ab Purified from bioreactor concentrate by Protein A/G.

Immunogène: A recombinant fragment (around aa 376-502) of human SILV protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: SK-MEL-28 cells. Melanoma.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Melanoma gp100

Antigène: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Autre désignation: SILV

Sujet:

95 kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, Premelanosome protein (PMEL17), Secreted melanoma-associated ME20 antigen, SILV, Silver homolog

CACO-38 or SK-MEL-28 cells. Melanoma.

Poids moléculaire: 26-100kDa

ID gène: 6490, 95972

UniProt: P40967