SOX2 anticorps (AA 176-305)

N° du produit ABIN6940611

Cet anticorps anti-SOX2 est un anticorps Souris Monoclonal détectant SOX2 dans WB, ELISA, IHC, Coat et StM. Adapté pour Humain et Souris.

Aperçu rapide pour SOX2 anticorps (AA 176-305) (ABIN6940611)

Antigène: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SOX2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Coating (Coat), Staining Methods (StM)

Clone: SOX2-1791

Détail du produit anti-SOX2 anticorps

Épitope: AA 176-305

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment (within aa176-305) of human SOX2 protein (exact sequence is proprietary)

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Positive Control: A549, NCCIT cells. Lung or Cervicalcarcinoma.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), ,Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur SOX2

Antigène: SOX2 (SRY (Sex Determining Region Y)-Box 2 (SOX2))

Autre désignation: SOX2

Sujet: SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.

Poids moléculaire: 34kDa

ID gène: 6657

UniProt: P48431

Pathways: Dopaminergic Neurogenesis, Sensory Perception of Sound, Stem Cell Maintenance, Cell RedoxHomeostasis