Spectrin beta Chain, Erythrocyte (SPTB) anticorps

N° du produit ABIN6940649

Cet anticorps Souris Monoclonal détecte spécifiquement dans WB, IF, IHC, ELISA, Coat et StM. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour Spectrin beta Chain, Erythrocyte (SPTB) anticorps (ABIN6940649)

Antigène: Spectrin beta Chain, Erythrocyte (SPTB)

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Clone: RG-26

Détail du produit

Purification: Purified by Protein A/G

Immunogène: Red cell membranes

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Positive Control: Jurkat whole cell lysate (WB). Human colon carcinoma tissue (IHC).

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), ,Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détail du antigène

Antigène: Spectrin beta Chain, Erythrocyte (SPTB)

Autre désignation: SPTB

Sujet: Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death.

Poids moléculaire: 246kDa

ID gène: 6710

UniProt: O15020

Pathways: Regulation of Actin Filament Polymerization