Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) anticorps

N° du produit ABIN6940654

L’anticorps anti-Spectrin, Beta, Non-erythrocytic 2 Monoclonal Souris est utilisé pour la détection de Spectrin, Beta, Non-erythrocytic 2 dans des échantillons de Humain. Il a été validé pour WB, IHC et IF.

Aperçu rapide pour Spectrin, Beta, Non-erythrocytic 2 (SPTBN2) (AA 356-475) anticorps (ABIN6940654)

Antigène: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: SPTBN2-1778

Détail du produit

Épitope: AA 356-475

Fonction: Spectrin beta III (SPTBN2) Antibody

Specificité: Spectrin is an actin binding protein that is a major component of the plasma membrane skeleton. Spectrins function as membrane organizers and stabilizers by forming dimers, tetramers and higher polymers. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin °™ and spectrin æ are present in erythrocytes, whereas spectrin °™ II (also designated fodrin °™) and spectrin æ I (also designated fodrin æ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin æ III is highly homologous to both spectrin æ I and spectrin æ II. Spectrin æ III is highly expressed in brain, kidney, pancreas and liver, and at lower levels in lung and placenta. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment (around aa356-475) of human SPTBN2 protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: PANC-1, HeLa, PC-3 cells. Brain, Pancreas or Liver.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détail du antigène

Antigène: Spectrin, Beta, Non-erythrocytic 2 (SPTBN2)

Autre désignation: SPTBN2

Sujet:

Beta III spectrin, SCA5, Spectrin beta chain brain 2, Spectrin beta non-erythrocytic 2, Spectrin non-erythroid beta chain 2, Spinocerebellar ataxia 5, SPTBN2

HeLa or PC-3 cells. Human brain, PANC-1, pancreas or liver.

Poids moléculaire: 246kDa

ID gène: 6712, 26915

UniProt: O15020

Pathways: Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis