NKX2-1 anticorps

N° du produit ABIN6940741

Cet anticorps anti-NKX2-1 est un anticorps Souris Monoclonal détectant NKX2-1 dans IHC, FACS, IF et StM. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour NKX2-1 anticorps (ABIN6940741)

Antigène: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NKX2-1 est non-conjugé

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Clone: 8G7G3-1

Détail du produit anti-NKX2-1 anticorps

Specificité: Recognizes a protein of 40 kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates.Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.

Réactivité croisée (Details): Shows a broad species reactivity.

Purification: Purified by Protein A/G

Immunogène: Rat full length TTF-1 recombinant protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: MAD109, MLE-15, H441-4 or H345 cells. Normal thyroid or lung.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur NKX2-1

Antigène: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Autre désignation: NKX2-1

Poids moléculaire: 40kDa

ID gène: 7080

UniProt: P43699

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Glucan Metabolic Process, Feeding Behaviour