Topoisomerase II alpha anticorps (AA 1352-1493)

N° du produit ABIN6940773

Cet anticorps Souris Monoclonal détecte spécifiquement Topoisomerase II alpha dans WB, IHC, IF et StM. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour Topoisomerase II alpha anticorps (AA 1352-1493) (ABIN6940773)

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Topoisomerase II alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Clone: TOP2A-1361

Détail du produit anti-Topoisomerase II alpha anticorps

Épitope: AA 1352-1493

Specificité: It recognizes a 170 kDa protein, which is identified as topoisomerase II is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

Purification: Purified by Protein A/G

Immunogène: Recombinant human Topoisomerase II alpha fragment (aa1352-1493)

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Positive Control: HeLa cells. Tonsil.

Known Application: Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Topoisomerase II alpha

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Autre désignation: TOP2A

Poids moléculaire: 170kDa

ID gène: 7153

UniProt: P11388

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases