Recombinant GRP94 anticorps

N° du produit ABIN6940801

L’anticorps anti-GRP94 Monoclonal Lapin est utilisé pour la détection de GRP94 dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Poulet, Hamster, Chien, Lapin, Porc, Cobaye, Singe, Mouton, Cheval et Xenopus laevis. Il a été validé pour WB, IHC et FACS.

Aperçu rapide pour Recombinant GRP94 anticorps (ABIN6940801)

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poulet, Hamster, Chien, Lapin, Porc, Cobaye, Singe, Mouton, Cheval, Xenopus laevis

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp GRP94 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: HSP90B1-3168R

Détail du produit anti-GRP94 anticorps

Fonction: Recombinant GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Antibody

Specificité: Recognizes a protein of 94 kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant full-length human HSP90B1 protein

Isotype: IgG, kappa

Information d'application

Indications d'application:

Positive Control: HepG2, U20S cells. About 50 % of breast tumors express grp94 protein.

Known Application: Flow Cytometry (0.5-1 μg/million cells),Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur GRP94

Antigène: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Autre désignation: HSP90B1

Sujet:

Glucose Regulated Protein 94, 94 kDa glucose-regulated protein, Endoplasmin, Endothelial cell (HBMEC) glycoprotein (ECGP), Heat shock protein 90 kDa beta member 1 (HSP90B1), Stress inducible tumor rejection antigen GP96, TRA1, Tumor rejection antigen-1 (gp96)

HePG2, U20S cells. About 50 % of breast tumors express grp94 protein. A431, A549, Pancreas

Poids moléculaire: 94kDa

ID gène: 7184, 192374

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades