C4B anticorps

N° du produit ABIN6940809

Cet anticorps anti-C4B est un anticorps Souris Monoclonal détectant C4B dans IHC, IF et StM. Adapté pour Humain.

Aperçu rapide pour C4B anticorps (ABIN6940809)

Antigène: C4B (C4b) (Complement Component C4b (C4b))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp C4B est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)

Clone: SPM545

Détail du produit anti-C4B anticorps

Specificité: This MAb is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 Molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.

Purification: Purified by Protein A/G

Immunogène: Recombinant human Complement 4d protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Rejected Renal Transplant Tissue.

Known Application: Immunofluorescence (1:50-1:100), Immunohistochemistry (Formalin-fixed) (1:200-1:400 for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur C4B

Antigène: C4B (C4b) (Complement Component C4b (C4b))

Autre désignation: C4B

Poids moléculaire: 192kDa (predicted)

ID gène: 720, 721

Pathways: Système du Complément