UCHL1 anticorps

N° du produit ABIN6940850

Cet anticorps Souris Monoclonal détecte spécifiquement UCHL1 dans WB, IHC, IF et FACS. Il présente une réactivité avec des échantillons de Humain, Rat, Souris, Boeuf (Vache), Chien et Hamster.

Aperçu rapide pour UCHL1 anticorps (ABIN6940850)

Antigène: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Chien, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp UCHL1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 31A3

Détail du produit anti-UCHL1 anticorps

Fonction: PGP9.5 / UchL1 (pan-Neuronal Marker) Antibody

Specificité: This MAb reacts with a protein of 20-30 kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Native UchL1 (PGP9.5) protein from brain

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: T98G cells. Cerebellum.

Known Application: Flow Cytometry (1-2 μg/million cells),Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur UCHL1

Antigène: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Autre désignation: UCHL1

Sujet:

Gracile Axonal Dystrophy, Neuron Cytoplasmic Protein 9.5, Park5, Parkinson Disease 5, PGP95, Protein Gene Product 9.5, Ubiquitin Carboxyl-terminal Esterase L1, Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1, Ubiquitin Thioesterase L1, Ubiquitin Thiolesterase L1

Human brain, T98G cells. Cerebellum. Mouse Brain, Rat Brain or Hamster Brain

Poids moléculaire: 20-30kDa

ID gène: 7345, 518731

UniProt: P09936

Pathways: Feeding Behaviour