XRCC3 anticorps

N° du produit ABIN6940907

Cet anticorps anti-XRCC3 est un anticorps Souris Monoclonal détectant XRCC3 dans WB, IHC et StM. Adapté pour Humain.

Aperçu rapide pour XRCC3 anticorps (ABIN6940907)

Antigène: XRCC3 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp XRCC3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Clone: 10F1-6

Détail du produit anti-XRCC3 anticorps

Purification: Purified by Protein A/G

Immunogène: Recombinant full-length human XRCC3 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Human placenta. HeLa nuclear extract.

Known Application: Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur XRCC3

Antigène: XRCC3 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3))

Autre désignation: XRCC3

Sujet: The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad51. XRCC1 is a protein that facilitates the DNA base excision repair pathway by interacting with DNA ligase III and DNA polymerase to repair DNA single-strand breaks. XRCC2 and XRCC3 are both involved in maintaining chromosome stability during cell division. XRCC2 is required for efficient repair of DNA double-strand breaks by homologous recombination between sister chromatids, and XRCC3 interacts directly with Rad51 to cooperate with Rad51 during recombinational repair. XRCC4 is an accessory factor of DNA Ligase IV that preferentially binds DNA with nicks or broken ends. XRCC4 binds to DNA Ligase IV and enhances its joining activity, and it is also involved in V(D)J recombination. Any defect in one of the known components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80, DNA-PKCS, XRCC4 and DNA Ligase IV) leads to abortion of the V(D)J rearrangement process and early block in both T and B cell maturation.

Poids moléculaire: 40kDa

ID gène: 7517

UniProt: O43542

Pathways: Réparation de l'ADN