Caldesmon anticorps

N° du produit ABIN6940940

Cet anticorps Souris Monoclonal détecte spécifiquement Caldesmon dans IHC et StM. Il présente une réactivité envers Humain.

Aperçu rapide pour Caldesmon anticorps (ABIN6940940)

Antigène: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Caldesmon est non-conjugé

Application: Immunohistochemistry (IHC), Staining Methods (StM)

Clone: CALD1-820

Détail du produit anti-Caldesmon anticorps

Specificité: Recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120'150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70'80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Aucune reactivité croisée: Rat (Rattus)

Purification: Purified by Protein A/G

Immunogène: Recombinant full-length human CALD1 protein

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma.

Known Application: Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Caldesmon

Antigène: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Autre désignation: CALD1

Poids moléculaire: 150kDa

ID gène: 800

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction