Cyclin E1 anticorps (AA 10-176)

N° du produit ABIN6940994

Détail du produit anti-Cyclin E1 anticorps

Antigène: Cyclin E1 (CCNE1)

Épitope: AA 10-176

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin E1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Purification: Purified by Protein A/G

Immunogène: Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176) (exact sequence is proprietary)

Clone: CCNE1-2460

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Positive Control: Jurkat, MCF, A431, HeLa cells. Breast or Colon Carcinoma.

Known Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Cyclin E1

Antigène: Cyclin E1 (CCNE1)

Autre désignation: CCNE1 (CCNE1 Produits)

Synonymes: anticorps CCNE1, anticorps ccne, anticorps cyclinE, anticorps CCNE, anticorps AW538188, anticorps CycE1, anticorps CYCLE, anticorps Ccne, anticorps cyce2, anticorps cycE3, anticorps cyce, anticorps fa18e07, anticorps wu:fa18e07, anticorps cyclin E1, anticorps cyclin E1 L homeolog, anticorps cyclin E1 S homeolog, anticorps CCNE1, anticorps ccne1, anticorps Ccne1, anticorps ccne1.L, anticorps ccne1.S

Sujet: Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.

Poids moléculaire: 53kDa

ID gène: 898

UniProt: P24864

Pathways: Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases