CD22 anticorps (AA 52-178)

N° du produit ABIN6941120

Cet anticorps Souris Monoclonal détecte spécifiquement CD22 dans FACS, WB, ELISA, IHC, IF, Coat et StM. Il présente une réactivité envers Humain.

Aperçu rapide pour CD22 anticorps (AA 52-178) (ABIN6941120)

Antigène: CD22 (CD22 Molecule (CD22))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD22 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Coating (Coat), Staining Methods (StM)

Clone: BLCAM-1795

Détail du produit anti-CD22 anticorps

Épitope: AA 52-178

Specificité: Recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary)

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Raji, Ramos, MOLT4 and human peripheral blood lymphocytes. Tonsil, Lymph Node or Spleen.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA),Flow Cytometry (1-2 μg/million cells),Western Blot (1-2 μg/mL),Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD22

Antigène: CD22 (CD22 Molecule (CD22))

Autre désignation: CD22

Poids moléculaire: 130-140kDa

ID gène: 933

UniProt: P20273