CD34 anticorps

N° du produit ABIN6941168

L’anticorps Souris Monoclonal anti-CD34 a été validé pour FACS, IHC, IF, ISt et StM. Il convient pour détecter CD34 dans des échantillons de Humain, Cynomolgus et Macaque rhésus.

Aperçu rapide pour CD34 anticorps (ABIN6941168)

Antigène: CD34

Reactivité: Humain, Cynomolgus, Macaque rhésus

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD34 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunostaining (ISt), Staining Methods (StM)

Clone: QBEnd-10

Détail du produit anti-CD34 anticorps

Specificité: This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified intothree main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain.CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. It appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels 85 % of angiosarcoma and Kaposi's sarcoma, but with a lower specificity.

Aucune reactivité croisée: Boeuf (Vache), Chien, Rat (Rattus), Mouton

Purification: Purified by Protein A/G

Immunogène: Detergent solubilized vesicular suspension prepared from human term placenta

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Jurkat or KG-1 cells. Human tonsil or angiosarcoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD34

Antigène: CD34

Autre désignation: CD34

Poids moléculaire: 90-110kDa

ID gène: 947

UniProt: P28906