CD40 anticorps (AA 29-107)

N° du produit ABIN6941187

Cet anticorps Souris Monoclonal détecte spécifiquement CD40 dans FACS, ELISA, IHC, StM et Coat. Il présente une réactivité envers Humain.

Aperçu rapide pour CD40 anticorps (AA 29-107) (ABIN6941187)

Antigène: CD40

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD40 est non-conjugé

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Staining Methods (StM), Coating (Coat)

Clone: C40-2383

Détail du produit anti-CD40 anticorps

Épitope: AA 29-107

Purification: Purified by Protein A/G

Immunogène: Recombinant fragment of human CD40 protein (around aa 29-107) (exact sequence is proprietary)

Isotype: IgG2b kappa

Information d'application

Indications d'application:

Positive Control: U2OS, L-428 cells. Tonsil, Spleen or Thymus.

Known Application: ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD40

Antigène: CD40

Autre désignation: CD40

Sujet: CD40 is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. CD40 is expressed on B-lymphocytes, follicular dendritic cells, bone marrow-derived dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zones of secondary lymphoid organs.

Poids moléculaire: 43kDa

ID gène: 958

UniProt: P25942

Pathways: Signalisation NF-kappaB, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints