CD47 anticorps (AA 18-135)

N° du produit ABIN6941214

Cet anticorps anti-CD47 Monoclonal Souris (Clone CD47-2937) (ABIN6941214) détecte spécifiquement CD47 dans WB, IHC et ELISA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CD47 anticorps (AA 18-135) (ABIN6941214)

Antigène: CD47

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD47 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: CD47-2937

Détail du produit anti-CD47 anticorps

Épitope: AA 18-135

Fonction: CD47 / IAP (Integrin Associated Protein) Antibody

Specificité: This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: A recombinant fragment (around aa 18-135) of human CD47 protein (exact sequence is proprietary)

Isotype: IgG2c, kappa

Information d'application

Indications d'application:

Positive Control: MCF-7, HUVEC or OVCA3 cells (IF/FACS). Human placenta, brain or ovarian tumors (IHC).

Known Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL for 60 minutes at RT), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD47

Antigène: CD47

Autre désignation: CD47

Sujet:

Antigenic Surface Determinant Protein OA3, IAP, Integrin Associated Protein, Integrin Associated Signal Transducer, MER6, OA3, Protein MER6, Rh Related Antigen

MCF-7, Jurkat, THP-1 or U-87 cells. Human prostate.

Poids moléculaire: ~50kDa

ID gène: 961, 446414

UniProt: Q08722