CD47 anticorps (FITC)

N° du produit ABIN192129

Détail du produit anti-CD47 anticorps (FITC)

Antigène: CD47

Reactivité: Humain, Porc, Non-Human Primate

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD47 est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Specificité: The antibody MEM-122 reacts with an extracellular epitope of CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor, immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.

Réactivité croisée (Details): Non-Human Primates, Human, Porcine

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: COS-7 (African green monkey) cells

Clone: MEM-122

Isotype: IgM

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Commentaires:

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD47 (FITC) (ABIN192129)

Smith, Patel, Seatter, Deehan, Brown, Brooke, Goodridge, Howard, Rigley, Harnett, Harnett: "A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes." dans: Blood, Vol. 102, Issue 7, pp. 2532-40, (2003) (PubMed).

Détails sur CD47

Antigène: CD47

Autre désignation: CD47 (CD47 Produits)

Synonymes: anticorps IAP, anticorps MER6, anticorps OA3, anticorps 9130415E20Rik, anticorps AA407862, anticorps AI848868, anticorps AW108519, anticorps B430305P08Rik, anticorps Itgp, anticorps CD47/IAP, anticorps alkaline phosphatase, intestinal, anticorps CD47 molecule, anticorps CD47 antigen (Rh-related antigen, integrin-associated signal transducer), anticorps Cd47 molecule, anticorps ALPI, anticorps CD47, anticorps Cd47

Sujet: CD47 Molecule,CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages, this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.,IAP, OA3, MER6

ID gène: 961

UniProt: Q08722