CD68 anticorps (AA 150-301)
Aperçu rapide pour CD68 anticorps (AA 150-301) (ABIN6941245)
Antigène
Voir toutes CD68 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 150-301
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Specificité
- This antibody recognizes a glycoprotein of 110 kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
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Purification
- Purified by Protein A/G
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Immunogène
- Recombinant fragment of humanCD68 protein (around aa 150-301) (exact sequence is proprietary)
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Isotype
- IgG2b kappa
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Indications d'application
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Positive Control: U87MG, Tonsil, Lymph Node or Spleen.
Known Application: Flow Cytometry (1-2 μg/million cells),ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
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Restrictions
- For Research Use only
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Concentration
- 200 μg/mL
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Buffer
- 10 mM PBS with0.05 % BSA & 0.05 % azide.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-80 °C
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Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Date de péremption
- 24 months
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- CD68 (CD68 Molecule (CD68))
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Autre désignation
- CD68
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Poids moléculaire
- 110kDa
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ID gène
- 968
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UniProt
- P34810
Antigène
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