CD74 anticorps

N° du produit ABIN6941253

L’anticorps anti-CD74 Monoclonal Souris est utilisé pour la détection de CD74 dans des échantillons de Humain, Souris et Babouin. Il a été validé pour FACS, WB, IHC et IF.

Aperçu rapide pour CD74 anticorps (ABIN6941253)

Antigène: CD74 (HLA-DR-gamma (CD74))

Reactivité: Humain, Souris, Babouin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD74 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: LN-2

Détail du produit anti-CD74 anticorps

Fonction: CD74 (B-Cell Marker) Antibody

Specificité: It recognizes a protein of ~35 kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

Aucune reactivité croisée: Rat (Rattus)

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogène: SU-DHL-4 lymphoma cells

Isotype: IgG1, kappa

Information d'application

Indications d'application:

Positive Control: Daudi or Raji cells. Tonsil or lymph node.

Known Application: Flow Cytometry (0.5-1 μg/million cells), (Methanol Fixed)Immunofluorescence (1-4 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD74

Antigène: CD74 (HLA-DR-gamma (CD74))

Autre désignation: CD74

Sujet:

CLIP, DHLAG, Gamma chain of class II antigens, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, HLADR-gamma (HLADG), Ia antigen-associated invariant chain, la-gamma, Major histocompatibility complex class II invariant chain, MHC HLA-DR gamma chain

Daudi or Raji cells. Tonsil or lymph node.

Poids moléculaire: 33-41kDa

ID gène: 972, 436568

UniProt: P044233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints