CD81 anticorps

N° du produit ABIN6941280

L’anticorps Souris Monoclonal anti-CD81 a été validé pour FACS, WB, IHC, IF, Func et StM. Il convient pour détecter CD81 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour CD81 anticorps (ABIN6941280)

Antigène: CD81

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD81 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Functional Studies (Func), Staining Methods (StM)

Clone: 1-3-3-22

Détail du produit anti-CD81 anticorps

Specificité: Recognizes a protein of 26 kDa, identified as CD81 (Workshop VI, Code CD81.1)). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Purification: Purified by Protein A/G

Immunogène: B-Cell line derived from a Burkitt lymphoma

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Ramos, Daudi, Raji, U266, HUT-78, HUT-102, Jurkat, HL-60, KG1a, A293, A431, MG63 and human lymphocytes. Tonsil or Liver.

Known Application: Functional Studies (Order Ab without Azide),Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1.0 μg/mL for 2 hours at RT),Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur CD81

Antigène: CD81

Autre désignation: CD81

Poids moléculaire: 26kDa

ID gène: 975

UniProt: P60033

Pathways: Inositol Metabolic Process, Hepatitis C