Recombinant BrdU anticorps
Aperçu rapide pour Recombinant BrdU anticorps (ABIN6941313)
Antigène
Voir toutes BrdU AnticorpsType d'anticorp
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
-
-
Specificité
- It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
-
Purification
- Purified by Protein A/G
-
Immunogène
- Bromodeoxyuridine (BrdU) conjugated to BSA
-
Isotype
- IgG1 kappa
-
-
-
-
Indications d'application
-
Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.
Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT) [For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC Optimal dilution for a specific application should be determined.
-
Restrictions
- For Research Use only
-
-
-
Concentration
- 200 μg/mL
-
Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
-
Agent conservateur
- Sodium azide
-
Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Stock
- 4 °C,-80 °C
-
Stockage commentaire
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
-
Date de péremption
- 24 months
-
-
- BrdU (Bromodeoxyuridine (BrdU))
-
Autre désignation
- Bromodeoxyuridine (BrdU) (Proliferation Marker)
-
Classe de substances
- Chemical
Antigène
-