Keratin 77 anticorps

N° du produit ABIN6941429

Cet anticorps anti-Keratin 77 est un anticorps Souris Monoclonal détectant Keratin 77 dans IHC, WB, IF, FACS et StM. Adapté pour Humain, Rat, Boeuf (Vache), Chien, Souris, Lapin, Poulet, Singe et Tortue.

Aperçu rapide pour Keratin 77 anticorps (ABIN6941429)

Antigène: Keratin 77 (KRT77)

Reactivité: Humain, Rat, Boeuf (Vache), Chien, Souris, Lapin, Poulet, Singe, Tortue

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Keratin 77 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Clone: AE-1

Détail du produit anti-Keratin 77 anticorps

Specificité: This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Purification: Purified by Protein A/G

Immunogène: Human epidermal keratin

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Positive Control: Skin or squamous cell carcinoma (IHC).

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Keratin 77

Antigène: Keratin 77 (KRT77)

Autre désignation: KRT77

Poids moléculaire: 40-56.5kDa

ID gène: 374454

UniProt: Q7Z794