AGO2 anticorps

N° du produit ABIN6942129

Détail du produit anti-AGO2 anticorps

Antigène: AGO2 (Argonaute 2 (AGO2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp AGO2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Réactivité croisée: Humain, Souris, Rat

Purification: Purified by Protein A.

Immunogène: Human Argonaute 2 between 1 to 150 amino acids

Clone: 7C3

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC()

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C for 12 months.

Date de péremption: 12 months

Détails sur AGO2

Antigène: AGO2 (Argonaute 2 (AGO2))

Autre désignation: Argonaute 2 (AGO2 Produits)

Synonymes: anticorps EIF2C2, anticorps T19E23.7, anticorps T19E23_7, anticorps argonaute 2, anticorps Q10, anticorps Eif2c2, anticorps Argonaute2, anticorps eif2c1, anticorps eif2c2, anticorps 1110029L17Rik, anticorps 2310051F07Rik, anticorps AI225898, anticorps AL022874, anticorps AW546247, anticorps ENSMUSG00000072493, anticorps Gerp95, anticorps Gm10365, anticorps mKIAA4215, anticorps AGO2, anticorps AG02, anticorps AGO 2, anticorps Ago-2, anticorps Ago2, anticorps CG13452, anticorps CG7439, anticorps Dm Ago2, anticorps Dmel\\CG7439, anticorps ago, anticorps ago-2, anticorps ago2, anticorps dAGO2, anticorps dAgo2, anticorps eIF2C2, anticorps argonaute 2, RISC catalytic component, anticorps Argonaute family protein, anticorps argonaute 2, RISC catalytic component L homeolog, anticorps argonaute RISC catalytic subunit 2, anticorps argonaute RISC catalytic component 2, anticorps Argonaute 2, anticorps AGO2, anticorps Ago2, anticorps ago2.L, anticorps ago2

Sujet:

Synonyms: Protein argonaute-2, Argonaute RISC catalytic component 2, Eukaryotic translation initiation factor 2C 2, PAZ Piwi domain protein, Protein slicer, Argonaute2, hAgo2, eIF-2C 2, eIF2C 2, PPD, AGO2, EIF2C2

Background: Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.

ID gène: 27161

UniProt: Q9UKV8

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization