CHAF1B anticorps

N° du produit ABIN6942548

Cet anticorps anti-CHAF1B est un anticorps Lapin Monoclonal détectant CHAF1B dans WB, IP, IHC (p), FACS, IF (cc) et IF (p). Adapté pour Humain.

Aperçu rapide pour CHAF1B anticorps (ABIN6942548)

Antigène: CHAF1B (Chromatin Assembly Factor 1, Subunit B (p60) (CHAF1B))

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp CHAF1B est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: 9A7

Détail du produit anti-CHAF1B anticorps

Réactivité croisée: Humain

Purification: Purified by Protein A.

Immunogène: Recombinant protein corresponding to the C-terminus of Human p60 CAF1.

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
IP(1-2 μg)

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C for 12 months.

Date de péremption: 12 months

Détails sur CHAF1B

Antigène: CHAF1B (Chromatin Assembly Factor 1, Subunit B (p60) (CHAF1B))

Autre désignation: CHAF1B

Sujet:

Synonyms: Chromatin assembly factor 1 subunit B, Chromatin assembly factor I p60 subunit, M-phase phosphoprotein 7, CAF-1 subunit B, CAF-I 60 kDa subunit, CAF-I p60, CHAF1B, CAF1A, CAF1P60, MPHOSPH7, MPP7, p60 CAF1

Background: Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin-associated proteins 1 (HP1_, HP1_ and HP1_). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

ID gène: 8208

UniProt: Q13112