MSH6 anticorps

N° du produit ABIN6944121

Cet anticorps Lapin Monoclonal détecte spécifiquement MSH6 dans WB, IHC (p) et IF (cc). Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour MSH6 anticorps (ABIN6944121)

Antigène: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp MSH6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 3G4

Détail du produit anti-MSH6 anticorps

Réactivité croisée: Humain, Souris

Purification: Purified by Protein A.

Immunogène: C terminal Human MSH6

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C for 12 months.

Date de péremption: 12 months

Détails sur MSH6

Antigène: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Autre désignation: MSH6

Sujet:

Synonyms: DNA mismatch repair protein Msh6, G/T mismatch-binding protein, MutS protein homolog 6, MutS-alpha 160 kDa subunit, hMSH6, GTBP, GTMBP, p160, MSH6

Background: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

ID gène: 2956

UniProt: P52701

Pathways: Réparation de l'ADN, Chromatin Binding, Production of Molecular Mediator of Immune Response